Our consultations and services are detailed as follows:

Mass Spectrometry Imaging

  • Regular tissue freezing with liquid nitrogen conserving tissue shape and integrity
  • Cryosectioning of frozen and FFPE tissues
  • Sample preparation for MALDI imaging including regular washing, desalting, and lipid/protein/dye removal.
  • MALDI matrix purification and solution preparation
  • Robotic matrix deposition on tissue sections using HTX M5 sprayer
  • MALDI imaging of various tissue types at high spatial resolution (20 um) and super high speed (40 pixels per second).
  • On-tissue trypsin digestion for protein identification and proteomics studies
  • MALDI MS/MS for compound/protein identification
  • Targeted small molecule/drug imaging and identification
  • Comprehensive data analysis such as heat map visualization, pathology-guided IMS, and statistical analysis

Mass Spectrometry-Based High-Throughput Screening

  • Preparation of enzymatic reaction such as enzyme/substrate stock handling and preparation, enzyme/small molecule dispensing and incubation, reaction quenching and aliquoting.
  • Generating dose-response source plates with serial dilutions up to 16 different concentrations
  • Rigorous data analysis including IC50 calculations, single point and DR curve plotting
  • Z’ calculations for plate-to-plate variations
  • Hit identification

Proteomics and Quantitative Mass Spectrometry

  • Tryptic and peptic digestion of proteins, both in solution on-gel digestion
  • LC/MS of intact proteins
  • LC/MS/MS for protein identification and post-translational modification (PTM) analysis
  • Site-specific modifications of proteins such as lysine acetylation and methylation.
  • MRM and PRM assays for metabolite, drug, and protein quantitation in biological samples such as plasma and cell culture.

To request our services, please complete our service request form.