The following services are available for the Lentiviral Laboratory.
To schedule for services, please contact Dr. Liana Asatryan at asatryan@usc.edu
Services provided include
- Backbone vectors for cDNA expression
- Backbone vectors for shRNA expression
- Development of lentiviral constructs
- Small-scale lentivirus production
- Transductions of primary cells and tissue culture
- Production of stable cell lines
- Consultation for vector modalities, troubleshooting, support documentation for grants
Services turnaround time
- Lentiviral vector construction – 6-8 weeks
- Lentivirus packaging and concentration (small scale) – 10 days
- Lentiviral stock titrations – 2 weeks
- Lentiviral infections of primary cells including neurons – 2-4 days
Backbone vectors for cDNA expression
- pLVX-AcGFP1-C1
- pLVX-AcGFP1-N1
- pLVX-IRES-ZsGreen1
- pLVX-IRES-ZsGreen1-C1
- pLVX-IRES-ZsGreen1-N1
Backbone vectors for shRNA expression
- pLVX-shRNA1
- pLVX-shRNA2